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Literature about putative epigenetic state changes in mammal sequences after cloning steps in Escherichia coli

Literature about putative epigenetic state changes in mammal sequences after cloning steps in Escherichia coli



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I would like you to point me out some literature about putative epigenetic state changes in mouse/mammal sequences after cloning steps in Escherichia coli.

This are the last search details I used in NCBI PubMed:

"epigenomics"[MeSH Terms] AND "cloning, molecular"[MeSH Terms] OR "molecular cloning"[All Fields] AND "sequence"[All Fields] AND ("escherichia coli"[All Fields] OR "e. coli"[All Fields])


E.coli strains that have dam or dcm methylases can methylate plasmid at adenines or cytosines respectively. See here - It is a NEB web page but has links to the cited references.

DH5α has both the methylases- dam+ dcm+ , BL21 is dam+ dcm and ET12567 is dam dcm


Watch the video: Epigenetics: Nature vs nurture (August 2022).