We are searching data for your request:
Upon completion, a link will appear to access the found materials.
I would like you to point me out some literature about putative epigenetic state changes in mouse/mammal sequences after cloning steps in Escherichia coli.
This are the last search details I used in NCBI PubMed:
"epigenomics"[MeSH Terms] AND "cloning, molecular"[MeSH Terms] OR "molecular cloning"[All Fields] AND "sequence"[All Fields] AND ("escherichia coli"[All Fields] OR "e. coli"[All Fields])
E.coli strains that have dam or dcm methylases can methylate plasmid at adenines or cytosines respectively. See here - It is a NEB web page but has links to the cited references.
DH5α has both the methylases- dam+ dcm+ , BL21 is dam+ dcm− and ET12567 is dam− dcm−